PRESS RELEASE
Bio-Botanica® announces Puresterol® (pueraria mirifica) based serums found to reduce wrinkles in clinical trials
Hauppauge, N.Y., November 9, 2023 – Bio-Botanica, Inc., ® the one American company that has
manufactured botanical extracts under one family for more than 50 years, has revealed the results of
clinical trials indicating its proprietary botanical extract ingredient, Puresterol® (Pueraria mirifica),
showed positive effects on the reduction of wrinkles when used in a face serum.
Pueraria mirifica grows only in the rich soils of the high-altitude rainforests of Thailand and has been
used by the Thais for a millennium as a rejuvenator and revitalizer to help offset some of the effects of
aging. Puresterol®, developed by Bio-Botanica, is the only patented, specially processed and highly
active compound of Pueraria mirifica available in the market today (US Patent # 7,658,955, 2023).
A third-party contract research organization (CRO) conducted clinical trials of Puresterol® in two
different serum formulations under sponsorship by Bio-Botanica. In each trial, 21 subjects in different
groups applied a serum in the morning and night for 28 days. The CRO used a state-of-the-art 3D
structured-light scanner and software to precisely measure the area, length, and depth of crow’s feet
and forehead wrinkles at day 0 and day 28. In addition, subjects were asked to evaluate their
assessment of their treatment.
The first, placebo-controlled trial indicated Puresterol® in a serum had a statistically significant greater
reduction of the depth of Crow’s feet wrinkles when compared to placebo (p<0.05). The Puresterol®
containing serum reduced the area of Crow’s feet wrinkles (p<0.01), the length of Crow’s feet wrinkles
(p<0.01), and the depth of forehead wrinkles (p<0.01) from day 0 to day 28 in a statistically significant
manner. 1 A follow up study with a different amount of Puresterol® in a serum indicated similar results
with a statistically significant reduction in area of Crow’s feet wrinkles (p<0.05), length of Crow’s feet
wrinkles (p<0.0001) and area of forehead wrinkles (p<0.05) from day 0 to day 28.
2 Additionally, over
80% of subjects in each of the Puresterol® groups indicated that at day 28, the Puresterol® based
serum left the contours of their face appearing smoother. These results support a previous in vitro trial
sponsored by Bio-Botanica® which found varied concentrations of Puresterol® inhibited Collagenase
activity accordingly.3 Collagenase is an enzyme known to break down collagen and elastin and possibly
lead to wrinkle formation.
Bio-Botanica® is a family-owned and operated company, specializing in botanical extracts since its
founding in 1972. Bio-Botanica® is a leading manufacturer of high-quality botanical extracts for
personal care/cosmetics, food and beverage, flavor/fragrance, supplements, and dietary supplement
industries. Located in Hauppauge, New York, Bio-Botanica’s ® 160,00 square foot botanical extraction
facility houses its own R&D, Microbiological, and QC laboratories. It has a state-of-the-art facility and
experienced staff who work together on every project ensuring that Bio-Botanica ® successfully meets
customer goals and objectives.
About Bio-Botanica, Inc.
Bio-Botanica® is a family-owned and operated company, specializing in botanicals extracts since its
founding in 1972. Bio-Botanica® is a leading manufacturer of high-quality botanical extracts for
personal care/cosmetics, food and beverage, flavor/fragrance, supplements, and nutraceutical
industries. Located in Hauppauge, New York, Bio-Botanica’s ® 160,00 square foot botanical extraction
facility houses its own R&D, Microbiological, and QC laboratories. It has a state-of-the-art facility and
experienced staff who work together on every project ensuring that Bio-Botanica ® successfully meets
customer goals and objectives.
